Molecule

Tris 

154563 

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Buffer and primary pH standard. pKa=8.3 at 20°C.1 Auxiliary material in pharmaceutical science.2,3
Packaging
1 kg in poly bottle
5 g in glass bottle
100, 500 g in poly bottle
5 kg in poly drum

T1410 

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

T4661 

Analysis Note
Not pyrogen tested. Certificates of analysis are available with lot specific information.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Packaging
Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

T1503 

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
General description
2222A 40% (w/w) solution in water is clear and colorless.
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Packaging
1 kg in poly bottle
25, 100, 250, 500 g in poly bottle
5, 10, 25, 50 kg in poly drum
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Protocols & Applications
Ensure correct concentrations of buffer solutions with the Buffer Calculator

T6066 

Application
Not tested for use in parenteral applications as specified in the EP monograph.
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

T1378 

General description
A 40% (w/w) solution in water is clear, colorless to faint yellow
Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Sigma 7-9 is a registered trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

T6791 

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

T6687 

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.

93350 

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

93362 

Other Notes
Running buffer component in non-denaturing agarose gel electrophoresis1; In non-denaturing and urea-denaturing polyacrylamide gel electrophoresis2; DNA digest analysis with capillary electrophoresis3.
Easily compare specifications for Trizma products with the Trizma specification table.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

08656 

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

93304 

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

93440 

Analysis Note
Exact concentration, expiry date, traceability and detailed information on certification can be found on the certificate and certification report, included in each package. Content and expiry date on label.
Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

04577 

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

T87602 

Packaging
1 kg in poly bottle
250 g in poly bottle
3 kg in poly drum

252859 

Other Notes
Easily compare specifications for Trizma products with the Trizma specification table.
Packaging
100, 500 g in poly bottle

33742 

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

30-5020 

Suitability
for electrophoresis

30-5025 

Suitability
for biological purposes

93349 

Other Notes
In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis1; Electrophoresis of proteins on cellulose acetate membranes2; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis3; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses4.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

21685 

Packaging
6 bottles for the preparation of 1 L ready-to-use solution (each)

93286 

Other Notes
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC

CHEBI:9754 

A primary amino compound that is tert-butylamine in which one hydrogen attached to each methyl group is replaced by a hydroxy group. A compound widely used as a biological buffer substance in the pH range 7--9; pKa = 8.3 at 20 degreeC; pKa = 7.82 at 37 degreeC.