Substance
ID:359736
Names and Identifiers
IUPAC name
2-amino-2-(hydroxymethyl)propane-1,3-diol
IUPAC Traditional name
tris buffer
Synonyms
2-Amino-2-(hydroxymethyl)-1,3-propanediolTrometamolTHAMTris base2-氨基-2-(羟甲基)-1,3-丙二醇Tris 碱氨基丁三醇三羟甲基氨基甲烷Trizma® baseTrizma® 碱Tris(hydroxymethyl)aminomethane
Properties
Product Information
Antion Traces
sulfate (SO42-): ≤5 mg/kg
chloride (Cl-): ≤5 mg/kg
Cation Traces
Al: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Pb: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Ba: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Mo: ≤5 mg/kg
Ni: ≤5 mg/kg
Sr: ≤5 mg/kg
As: ≤0.1 mg/kg
Bi: ≤5 mg/kg
Zn: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Li: ≤5 mg/kg
Na: ≤50 mg/kg
Useful pH Range
7 - 9
Ignition Residue
≤0.01% (as SO4)
Purity
≥99.8% (T)
Description
aminopeptidase substrate
Product Line
BioUltra
λ
4 M in H2O
Impurities
insoluble matter, passes filter test
Linear Formula
NH2C(CH2OH)3
Loss on Drying
≤0.5% loss on drying, 110 °C
Physical Property
Absorption Wavelength
λ: 280 nm Amax: 0.08
λ: 260 nm Amax: 0.10
Boiling Point
219-220 °C/10 mmHg(lit.)
Melting Point
168-172 °C
167-172 °C(lit.)
pH
10.5-12.0 (25 °C, 4 M in H2O)
Solubility
H2O: soluble4 M at 20 °C, clear, colorless
p𝘒ₐ
8.1 (25°C)
Safety Information
German water hazard class
2
GHS Signal Word
Warning
Safety Statements
26-36
MSDS Link
GHS Precautionary statements
P261-P305+P351+P338
Risk Statements
36/37/38
GHS Hazard statements
H315-H319-H335
RTECS
TY2900000
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
European Hazard Symbols
Irritant (Xi)Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Molecule Details
Other Notes
In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis1; Electrophoresis of proteins on cellulose acetate membranes2; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis3; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses4.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
In a high molar concentration for molecular weight determination of peptides and proteins by gel electrophoresis1; Electrophoresis of proteins on cellulose acetate membranes2; Calibration of polyacrylamide gel columns for the separation of oligonucleotides by capillary electrophoresis3; Determination of trace amounts of fluorine from a single sodium carbonate fusion of small geological sample masses4.
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Legal Information
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Molecular Spectra
No Data Available
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References
No Data Available
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