CAS 7365-45-9|2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid|4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid|monosodium salt, hepes|HEPES FREE ACID|N-2-Hydroxyethylpiperazine-N'-2-eth...

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acidHEPES FREE ACIDN-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acidHEPES solution4-(2-羟乙基)哌嗪-1-乙磺酸HEPESN-(2-羟乙基)哌嗪-N′-(2-乙磺酸)HEPES 溶液N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)HEPES 缓冲液HEPES buffered salineHEPES buffer solutionHEPES 缓冲盐溶液HEPES2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid羟乙基哌嗪乙磺酸4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acidHepes4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

Names and Identifiers

Registration numbers

CAS Number

EC Number

MDL Number

Beilstein Number

PubChem SID

248954142489579624895572248956161620884472488810624878774248787752487383326697267

PubChem CID

23831

Merck Index

144654

Protein Data Bank

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

CompTox Database

DTXSID5040382

MetaboLights Database

MTBLS265MTBLS49MTBLS1040MTBLS787MTBLS706MTBLS816MTBLS267MTBLS549MTBLS459MTBLS266MTBLS883MTBLS1540MTBLS263MTBLS264

ACToR Database

CHEMBL

Reaxys Registry

SureChEMBL Database

UniProt Database

P9WJN1O82833Q93ZN9A0R2W9

CHEBI ID

CHEBI:42332CHEBI:42334CHEBI:32949

Patent number

US2005027009EP1473299EP1595872US2007196384WO2006011809WO2006029634US2006148042US2008160542

PubMed Citation Links

22732654

PDBeChem Database

EPE

Registration numbers

Properties

Related Proteins

Related Proteins

Molecular Spectra

No Data Available

Click here to submit data

Molecule Details

MP Biomedicals

02101926

Crystalline
Free Acid
Zwitterionic Buffer useful in the pH range 6.8-8.2.
pKa=7.55 at 25°C

Sigma Aldrich

H4034

Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

H3375

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。
Protocols & Applications
Ensure correct concentrations of buffer solutions with the Buffer Calculator

H7523

Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

H0887

Caution
Concentrations of HEPES over 40mM may be toxic to some cell types.
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Application
HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture.

59205C

Application
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

54457

Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.

54459

Other Notes
One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation1,2; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates3; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials4.
Easily compare specifications for HEPES products with the HEPES specification table.

51558

Components
concentration (in H2O): 10 g/l HEPES; 16 g/l NaCl; 0.74 g/l KCl; 0.27 g/l Na2HPO4.2H2O; 2.0 g/l dextrose

13-2220

Suitability
for biological purposes

54461

Other Notes
Biological buffer1,2

ChEBI

CHEBI:42334

A HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. A Good's buffer substance, pKa = 7.55 at 20 degreeC.

Molecule Details

References

PubChem Literature

From Data Sources

• Biological buffer, pKa = 7.55 at 20^o: Biochemistry, 5, 467 (1966).

References

Bioactivity

PubChem BioAssay

Bioactivity

Molecule

ID:102506