CAS 7365-45-9|2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid|4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid|monosodium salt, hepes|HEPES FREE ACID|N-2-Hydroxyethylpiperazine-N'-2-eth...

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acidHEPES FREE ACIDN-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acidHEPES solution4-(2-羟乙基)哌嗪-1-乙磺酸HEPESN-(2-羟乙基)哌嗪-N′-(2-乙磺酸)HEPES 溶液N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)HEPES 缓冲液HEPES buffered salineHEPES buffer solutionHEPES 缓冲盐溶液HEPES2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid羟乙基哌嗪乙磺酸4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid4-(2-Hydroxyethyl)-1-piperazineethane sulfonic acidHepes4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

名称和标识

数据登录号

EC号

230-907-9231-791-2

CAS号

7365-45-9

PubChem SID

248957962489541424895572248956161620884472487877524888106248787742487383326697267

MDL号

Beilstein号

PubChem CID

默克索引号

PDB数据库

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

MetaboLights数据库

MTBLS49MTBLS265MTBLS1040MTBLS787MTBLS267MTBLS816MTBLS706MTBLS549MTBLS266MTBLS883MTBLS1540MTBLS263MTBLS264MTBLS459

CompTox数据库

CHEMBL

ACToR数据库

Reaxys Registry

CHEBI ID

CHEBI:42332CHEBI:42334CHEBI:32949

UniProt数据库

P9WJN1O82833A0R2W9Q93ZN9

专利号

EP1595872EP1473299US2005027009US2006148042WO2006029634US2008160542US2007196384WO2006011809

SureChEMBL数据库

SCHEMBL2417

PubMed文献链接

22732654

PDBeChem数据库

EPE

数据登录号

化合物性质

分子相关蛋白质

分子相关蛋白质

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描述信息

MP Biomedicals

02101926

Crystalline
Free Acid
Zwitterionic Buffer useful in the pH range 6.8-8.2.
pKa=7.55 at 25°C

Sigma Aldrich

H4034

Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

H3375

Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。
Protocols & Applications
Ensure correct concentrations of buffer solutions with the Buffer Calculator

H7523

Other Notes
使用 HEPES 规格表可以非常方便地比较 HEPES 产品的规格。

H0887

Caution
Concentrations of HEPES over 40mM may be toxic to some cell types.
Preparation Note
Prepared from HEPES free acid and cell culture grade water.
Application
HEPES is an organic zwitterionic buffering agent effective in the physiological pH range of 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. This product has been qualified for use in cell culture.

59205C

Application
HEPES is used in many media because it has more buffering capacity in the physiologic pH range of 7.2 - 7.4 at 37 C than sodium bicarbonate. It is commonly added at concentrations between 10 mM and 25 mM. Higher levels cause cytotoxicity. Because bicarbonate is nutritionally necessary for most cells, HEPES should be added in addition to, not in place of, sodium bicarbonate.

54457

Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.

54459

Other Notes
One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation1,2; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates3; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials4.
Easily compare specifications for HEPES products with the HEPES specification table.

51558

Components
浓度（溶于 H2O）：10g/L HEPES；16g/L NaCl；0.74g/L KCl；0.27g/L Na2HPO4.2H2O；2.0g/L 右旋糖

13-2220

Suitability
for biological purposes

54461

Other Notes
生物缓冲液1,2

ChEBI

CHEBI:42334

A HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. A Good's buffer substance, pKa = 7.55 at 20 degreeC.

描述信息

参考文献

PubChem文献

数据源提供

• Biological buffer, pKa = 7.55 at 20^o: Biochemistry, 5, 467 (1966).

参考文献

生物活性

PubChem BioAssay

生物活性

化合物信息

ID:102506