Substance
ID:351510
Names and Identifiers
IUPAC name
2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
Synonyms
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid4-(2-羟乙基)哌嗪-1-乙磺酸HEPESN-(2-羟乙基)哌嗪-N′-(2-乙磺酸)N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
IUPAC Traditional name
hepes
Registration numbers
CAS Number
EC Number
Beilstein Number
MDL Number
PubChem SID
Properties
Product Information
Cation Traces
Ca: ≤10 mg/kg
Ba: ≤5 mg/kg
Co: ≤5 mg/kg
Cu: ≤5 mg/kg
Pb: ≤5 mg/kg
As: ≤0.1 mg/kg
Bi: ≤5 mg/kg
Li: ≤5 mg/kg
Mn: ≤5 mg/kg
Al: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Sr: ≤5 mg/kg
Fe: ≤5 mg/kg
Cd: ≤5 mg/kg
Cr: ≤5 mg/kg
Mg: ≤5 mg/kg
K: ≤50 mg/kg
Zn: ≤5 mg/kg
Ni: ≤5 mg/kg
Useful pH Range
6.8 - 8.2
Antion Traces
sulfate (SO42-): ≤50 mg/kg
chloride (Cl-): ≤50 mg/kg
Impurities
phosphatases, none detected
insoluble matter, passes filter test
proteases, none detected
DNases, none detected
RNases, none detected
Grade
for molecular biology
Ignition Residue
≤0.1% (as SO4)
Purity
≥99.5% (T)
Product Line
BioUltra
λ
1 M in H2O
Empirical Formula (Hill Notation)
C8H18N2O4S
Loss on Drying
≤0.5% loss on drying, 110 °C
Physical Property
Absorption Wavelength
λ: 280 nm Amax: 0.035
λ: 260 nm Amax: 0.045
p𝘒ₐ
7.5 (25°C)
pH
5.0-6.5 (25 °C, 1 M in H2O)
Solubility
H2O: soluble1 M at 20 °C, clear, colorless
Safety Information
German water hazard class
1
MSDS Link
Personal Protective Equipment
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RTECS
TL6809000
Molecule Details
Other Notes
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.
3′-Terminal labeling of RNA with T4 RNA ligase1; Isolation and analysis of nuclear RNA - component of reaction buffer, prehybridization buffer, hybridization buffer2.
Easily compare specifications for HEPES products with the HEPES specification table.
Molecular Spectra
No Data Available
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References
No Data Available
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