Stabilized Chromogen Chromogen solution for immunohistology procedures and peroxidase and pseudo-peroxidase reactions. Ready-to-use directly as the final step in developing the color indicator in the enzyme reaction. Precipitates as a readily detected marker for immunohistopathological staining. Stable up to 15 months.
Other Notes 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol. Substrates Substrate for visualizing peroxidase conjugates in Western blotting.
Quantity Contains 30 mg substrate per tablet. Reconstitution Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use. Specificity 4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.