化合物信息

S2574  

Research Area: Infection
Biological Activity:
Tetracycline hydrochloride is a hydrochloride salt of tetracycline that is a broad-spectrum polyketide antibiotic. Tetracycline binds to the 30S subunit of microbial ribosomes. Tetracycline inhibits protein synthesis by blocking the attachment of charged aminoacyl-tRNA. Thus tetracycline prevents introduction of new amino acids to the nascent peptide chain. The action is usually inhibitory and reversible upon withdrawal of the drug. Resistance to the tetracycline results from changes in permeability of the microbial cell envelope. In susceptible cells, the tetracycline is concentrated from the environment and does not readily leave the cell. In resistant cells, the drug is not actively transported into the cell or leaves it so rapidly that inhibitory concentrations are not maintained. Tetracycline hydrochloride is used in the treatment of bacterial infections, such as urinary tract infections, acne, gonorrhea, chlamydia, and others.[1]References on Tetracycline HCl[1] http://en.wikipedia.org/wiki/Tetracycline, ,

T7660 

Application
Used in tetracycline controlled gene expression systems (gene switches) such a the tet-on and tet-off systems. Recommended for use in cell culture applications at 10 mg/L.
Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

T3383 

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.
包装
25, 100 g in poly bottle

T8032 

Application
建议以 10-20μg/ml 的量用于分子生物学应用。
Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.
Reconstitution
直接在样品瓶中用无菌水 (10mg/ml) 制备储存液。储存液在 2-8°C 下保存不应超过一周；四环素在水溶液中会发生水解。

T4062 

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.
Mode of Action: inhbit bacterial protein synthesis elongation at the level of aminoacyl-tRNA binding to the 30S ribosome. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria. Mode of Resistance: Loss of cell wall permeability.

T9823 

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.
Tetracycline inhibits bacterial protein synthesis elongation at the level of aminoacyl-tRNA binding to the 30S ribosome. Tetracycline passively diffuses through porin channels in the bacterial cell membrane. It also binds to the bacterial 50S ribosomal subunit which may alter the cytoplasmic membrane, causing intracellular components to leak from bacterial cells1. Mode of resistance is loss of cell wall permeability.
Application
Tetracycline is a broad spectrum polyketide antibiotic. It is used clinically to treat bacterial infections such as Rocky Mountain spotted fever, typhus fever, tick fevers, Q fever and Brill-Zinsser disease. It is used to treat upper respiratory infections and acne1. It is used to study multidrug resistance2 as well as potential side effects such as acute pancreatitis3. It has been used in target and resistance based mechanistic studies of novel antibiotics4.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

87130 

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.

31741 

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.
法律信息
VETRANAL 商标 Sigma-Aldrich Co. LLC

74749 

Biochem/physiol Actions
Mode of Action: Inhibits protein synthesis (elongation) by preventing binding of aminoacyl-tRNA to the 30S subunit. Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.Mode of Resistance: Loss of cell wall permeability.

T291400 

Antibiotic substance produced by Streptomyces spp. Antiamebic; antibacterial; antirickettsial.