化合物信息

DB00759 

T3258 

Application
Tetracycline is a broad spectrum polyketide antibiotic. It is used clinically to treat bacterial infections such as Rocky Mountain spotted fever, typhus fever, tick fevers, Q fever, and Brill-Zinsser disease. It is used to treat upper respiratory infections and acne1. It is used to study multidrug resistance2 as well as potential side effects such as acute pancreatitis3.
Biochem/physiol Actions
Tetracycline inhibits bacterial protein synthesis elongation at the level of aminoacyl-tRNA binding to the 30S ribosome. Tetracycline passively diffuses through porin channels in the bacterial cell membrane. It also binds to the bacterial 50S ribosomal subunit which may alter the cytoplasmic membrane, causing intracellular components to leak from bacterial cells1. Mode of resistance is via loss of cell wall permeability.

87128 

Other Notes
Review4; Inhibitor of protein synthesis, binds to the 30S subunit and inhibits binding of aminoacyl-tRNAs.
Application
Tetracycline is an inhibitor of protein synthesis and is used in tetracycline controlled gene expression systems (gene switches) such a the tet-on and tet-off systems 1. It is also used to select tetracyline resistant colonies in cell culture 2.
Biochem/physiol Actions
Tetracycline binds to the 30S subunit of the ribosome at the A-site. During protein biosynthesis, the new t-RNA with the amino acid attempts to bind to the A-site of the ribosome. However, since the A-site is blocked by the tetracycline, the aminoacyl-tRNA cannot bind to it. Without the attachment of the tRNA, protein biosynthesis cannot occur. Protein biosynthesis is inhibited and cell death occurs 3.

CHEBI:27902 

A broad-spectrum polyketide antibiotic produced by the Streptomyces genus of actinobacteria.