Substance
ID:350814
Names and Identifiers
IUPAC Traditional name
(5-bromo-4-chloro-1H-indol-3-yl)oxyphosphonic acid; P-toluidine
IUPAC name
4-methylaniline; [(5-bromo-4-chloro-1H-indol-3-yl)oxy]phosphonic acid
Synonyms
X-phosphate p-toluidine saltBCIP® 对甲苯胺盐5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt5-溴-4-氯-3-吲哚磷酸 对甲苯胺盐BCIP® p-toluidine salt
Properties
Product Information
Empirical Formula (Hill Notation)
C8H6BrClNO4P · C7H9N
Purity
≥99% (HPLC)
Safety Information
MSDS Link
German water hazard class
3
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
European Hazard Symbols
Irritant (Xi)GHS Precautionary statements
P261-P305+P351+P338
GHS Hazard statements
H315-H319-H335
Safety Statements
26
Storage Temperature
-20°C
GHS Signal Word
Warning
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Risk Statements
36/37/38
Molecule Details
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC
Molecular Spectra
No Data Available
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References
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