CAS 6578-06-9|5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE p-TOLUIDINE SALT|BCIP|X-phosphate|(5-bromo-4-chloro-1H-indol-3-yl)oxyphosphonic acid; P-toluidine|4-methylaniline; [(5-bromo-4-chloro-1H-indol-3-yl)...

General Information

Structure

Molecular Formula

C₁₅H₁₅BrClN₂O₄P

Molecular Mass

433.621361

Exact Mass

431.96413328

Charge

0

InChI

InChI=1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3

InChIKey

QEIFSLUFHRCVQL-UHFFFAOYSA-N

Canonic Smiles

Brc1ccc2c(c1Cl)c(c[nH]2)OP(=O)(O)O.Cc1ccc(cc1)N

Isomeric Smiles

Cc1ccc(N)cc1.OP(=O)(O)Oc1c[nH]c2c1c(Cl)c(Br)cc2

Calculated Properties

JChem

Acid pKa

1.726015

H Acceptors

3

H Donor

3

LogD (pH = 5.5)

0.10124553

LogD (pH = 7.4)

-0.6621499

Log P

2.4873745

Molar Refractivity

62.4259

Polarizability

25.400608

Polar Surface Area

82.55

Rotatable Bonds

2

Lipinski's Rule of Five

true

Data Source

Commercial Catalog

Academic Data

Data Source

Names and Identifiers

Synonyms

5-BROMO-4-CHLORO-3-INDOLYLPHOSPHATE p-TOLUIDINE SALTBCIPX-phosphate5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE p-TOLUIDINE SALTBCIP® 对甲苯胺盐5-Bromo-4-chloro-3-indolyl phosphate p-toluidine saltX-phosphate p-toluidine salt5-溴-4-氯-3-吲哚磷酸对甲苯胺盐BCIP® p-toluidine salt

IUPAC Traditional name

(5-bromo-4-chloro-1H-indol-3-yl)oxyphosphonic acid; P-toluidine

IUPAC name

4-methylaniline; [(5-bromo-4-chloro-1H-indol-3-yl)oxy]phosphonic acid

Names and Identifiers

Registration numbers

EC Number

229-506-1

CAS Number

6578-06-9

MDL Number

MFCD00040636

PubChem SID

24891823248915101620880472489207424850179

PubChem CID

81059

Registration numbers

Properties

Product Information

Properties

Related Proteins

No Data Available

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Molecular Spectra

No Data Available

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Molecule Details

MP Biomedicals

02100368

p-Toluidine Salt
A histochemical substrate for alkaline phosphatase

02193991

Molecular Biology Reagent
p-Toluidine Salt
Purity: >98%
A chromogenic substrate for alkaline phosphatase in ELISA.

Sigma Aldrich

B6777

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC

B0274

Quantity
Contains 25 mg substrate per tablet.
Specificity
Substrate of choice for use with alkaline phosphatase in immunoblotting and, less commonly, in immunohistological staining procedures. High assay sensitivity is achieved via amplification when BCIP is used in conjunction with Nitro Blue Tetrazolium (NBT) Tablets (Catalog No. N5514). This substrate produces an insoluble end product that is blue-purple in color and can be observed visually.
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC

B8503

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC

16670

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Molecule Details

References

PubChem Literature

From Data Sources

• Horwitz, J.P., et al., J. Med. Chem., 9 , 447 (1966).

References

Bioactivity

PubChem BioAssay

Bioactivity

Molecule

ID:102152