物质信息
ID:354312
名称和标识
别名
MTTMethylthiazolyldiphenyl-tetrazolium bromide3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromideThiazolyl Blue Tetrazolium Bromide
IUPAC标准名
2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-3H-1,2λ5,3,4-tetrazol-2-ylium bromide
IUPAC传统名
2-(dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-1,2λ5,3,4-tetrazol-2-ylium bromide
化合物性质
安全信息
GHS警示词
Warning
GHS危险声明
H315-H319-H335-H341
MSDS下载
个人保护装置
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
GHS警示性声明
P261-P281-P305+P351+P338
RTECS编号
XF8060000
德国WGK号
3
GHS危险品标识
呼吸道过敏，类别1
生殖细胞突变，类别1A，1B，2
致癌性，类别1A，1B，2
生殖毒性，类别1A，1B，2
特定目标器官毒性 – 一次接触，类别1,2
特定目标器官毒性 – 反复接触，类别1,2
吸入危险，类别1
急性毒性（口服，皮肤接触，吸入），类别4
皮肤刺激，类别2
眼刺激，类别2
皮肤过敏，类别1
特定目标器官毒性 -一次接触，类别3
欧盟危险性物质标志
刺激性 (Xi)危险公开号
36/37/38
安全公开号
26
产品相关信息
纯度
≥97.0% (enzymatic)
Empirical Formula (Hill Notation)
C18H16BrN5S
理化性质
熔点
195 °C (dec.)(lit.)
溶解度
methanol: soluble5 mg/mL, clear
描述信息
Other Notes
Review: Tetrazolium salts and formazans10
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
Review: Tetrazolium salts and formazans10
Application
Thiazolyl Blue Tetrazolium Blue (MTT) may be used in measurement of cell proliferation.1,2,3,4,5 MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent6 and for the detection of NAD.7 ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used.8 MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.9
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