Application Broadly used in molecular biology and cell culture as a selection agent for prokaryotic cells that have been transformed using the selectable marker gene (neo). Recommended for use in cell culture applications at 5 ml/L. Biochem/physiol Actions Mode of action: binds to the 30S and in some cases the 50S subunit causing miscoding; inhibits initiation and elongation during protein synthesis.Antimicrobial spectrum: Gram-negative and Gram-positive bacteria. Caution Stable at 37 °C for 5 days. Other Notes Formulated to contain 10 mg/ml neomycin in 0.9% sodium chloride. Protocols & Applications Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information