Biochem/physiol Actions
Mode of Action: Inhibits prokaryote protein synthesis. Binds to S12 protein of 30S ribosomal subunit, preventing the transition from initiation complex to chain-elongating ribosome, causing miscoding or inhibiting initiation. Mode of Resistance: Mutation in rpsL (gene for S12 ribosomal protein) prevents binding of streptomycin to ribosome. Aminoglycoside phosphotransferase also inactivates.Antimicrobial spectrum: Gram-negative and Gram-positive bacteria.
Application
Recommended for use in embryo culture at 50 mg/L. Stock solutions should be sterile-filtered and stored at 2-8 °C for up to a month or at -20 °C for extended periods. Solutions are stable at 37 °C for 3 days. Use to inhibit bacterial protein synthesis at the level of initiation. Use to study mechanisms of streptomycin resistance. Use together with penicillin and other agents to inhibit bacterial contamination in cell culture applications.