化合物信息

Ortho-Nitrophenyl-β-galactoside 

02102473 

Crystalline
Substrate for β-galactosidase

02194030 

Molecular Biology Reagent
Crystalline
Cell culture component for molecular genetics. Substrate for β-galactosidase

N1127 

包装
1 g in poly tube
250 mg in poly bottle
5, 25 g in poly bottle
500 mg in poly tube
Substrates
Chromogenic substrate for β-galactosidase
Application
2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.
Biochem/physiol Actions
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

73660 

Other Notes
Substrate for the determination of β-galactosidases1

CHEBI:90144 

A beta-D-galactoside having a 2-nitrophenyl substituent at the anomeric position.