Biochem/physiol Actions
Used to study the effect of expression, binding and inhibition of penicillin-binding proteins especially PBP2 on bacterial cell wall mucopeptide synthesis.
Cefotaxim inhibits bacterial cell wall synthesis by binding to penicillin-binding proteins (PBPs) which inhibits the final transpeptidation step of peptidoglycan synthesis in bacterial cell walls. As a result, bacteria lyse due to cell wall autolytic enzymes.
Other Notes
Broad spectrum third generation cephalosporin antibiotic.
Application
Cefotaxim is a broad spectrum third generation cephalosporin antibiotic that is used to study the effect of expression, binding and inhibition of penicillin-binding proteins especially PBP2 on bacterial cell wall mucopeptide synthesis. It has been used to find new residues involved in cefotaxime hydrolysis in CTX-M β-lactamases1, to study antibiotic-susceptible and -resistant Streptococcus pneumoniae infections 2, and to study pneumococcal pneumonia and the pharmokinetics of various treatments3.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information