Substance
ID:73966
Names and Identifiers
IUPAC name
3-[5-(2-fluorophenyl)-1,2,4-oxadiazol-3-yl]benzoic acid
Synonyms
AtalurenPTC124
IUPAC Traditional name
ataluren
Registration numbers
CAS Number
Properties
Safety Information
Storage Condition
-20°C
Physical Property
Solubility
DMSO
Product Information
Salt Data
Free Base
Molecule Details
Research Area
Description
Inflammation
Protocol
Kinase Assay [1]
Assays for read-through of LUC premature termination codons
Stock solutions of PTC124 (6 mM) are prepared in 100% DMSO HEK293 cells grown in a medium containing fetal bovine serum (FBS) and are stably transfected with LUC genes containing premature terminators at codon 190 (and 11 contextual variants thereof), treated with increasing concentrations of PTC124 for 16 hours, and assayed for luciferase activity. Luminescence of each culture relative to the control is quantified by standard procedures using a Viewlux CCD imager. Luminescence data are normalized to that produced with DMSO, and the fold suppression over background is calculated as (PTC124light units/DMSOlight units).
Cell Assay []
Animal Study [2]
Animal Models
Cftr-/- hCFTR-G542X transgenic mice
Formulation
Dissolved in DMSO, and diluted in saline
Doses
~60 mg/kg/day
Administration
Subcutaneous injection or oral administration
Molecular Spectra
No Data Available
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References
• Welch EM, et al. Nature, 2007, 447(7140), 87-91.
• Du M, et al. Proc Natl Acad Sci U S A, 2008, 105(6), 2064-2069.