Substance
ID:371516
Names and Identifiers
IUPAC name
(2S,3S)-1,4-disulfanylbutane-2,3-diol
Synonyms
DTT克莱兰氏试剂DL-二硫苏糖醇1,4-二巯基苏糖醇DL-DithiothreitolCleland’s reagentthreo-1,4-Dimercapto-2,3-butanediol
IUPAC Traditional name
dithiothreitol
Registration numbers
MDL Number
Beilstein Number
CAS Number
EC Number
Properties
Safety Information
Risk Statements
22-36/37/38
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
GHS Signal Word
Warning
European Hazard Symbols
Harmful (Xn)GHS Precautionary statements
P261-P305+P351+P338
RTECS
EK1610000
GHS Hazard statements
H302-H315-H319-H335
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Safety Statements
26-36
German water hazard class
3
Physical Property
Flash Point
235.4 °F
113 °C
Melting Point
41-44 °C(lit.)
Product Information
Purity
≥99.0%
Linear Formula
HSCH2CH(OH)CH(OH)CH2SH
Molecule Details
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Suitability
for biological purposes
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Suitability
for biological purposes
Molecular Spectra
No Data Available
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References
No Data Available
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