Substance
ID:363356
Names and Identifiers
IUPAC Traditional name
tetramethylbenzidine
IUPAC name
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
Synonyms
TMB Blotting PlusTMB substrateBM blueTMB3,3′,5,5′-四甲基联苯胺3,3′,5,5′-TetramethylbenzidineSure Blue TMB
Registration numbers
CAS Number
EC Number
MDL Number
Beilstein Number
PubChem SID
Properties
Safety Information
GHS Hazard statements
H315-H319-H335
RTECS
DV2300000
Risk Statements
36/37/38
European Hazard Symbols
Irritant (Xi)GHS Signal Word
Warning
MSDS Link
German water hazard class
3
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
GHS Precautionary statements
P261-P305+P351+P338
Safety Statements
26-36
Product Information
Grade
standard for GC
puriss. p.a.
Purity
≥99.0% (GC)
Shelf Life
(limited shelf life, expiry date on the label)
Linear Formula
[-C6H2(CH3)2-4-NH2]2
Physical Property
Melting Point
166-171 °C
168-171 °C(lit.)
Molecule Details
Application
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8
Other Notes
Standard for determining aromatic amines released from azo-dyes in commodities9
3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine1 suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood,2 assay of hemoglobin,3,4,5 assay of peroxidases.6,7,8
Other Notes
Standard for determining aromatic amines released from azo-dyes in commodities9
Molecular Spectra
No Data Available
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References
No Data Available
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