Substance

ID:351895

Names and Identifiers
Synonyms
CarbamideCarbonyldiamideUrea碳酰胺尿素碳酰二胺
IUPAC name
urea
IUPAC Traditional name
urea
Registration numbers
Beilstein Number
CAS Number
MDL Number
EC Number
PubChem SID
Properties
Product Information
Antion Traces
sulfate (SO42-): ≤10 mg/kg
chloride (Cl-): ≤5 mg/kg
Cation Traces
Ba: ≤5 mg/kg
Sr: ≤5 mg/kg
Li: ≤5 mg/kg
Zn: ≤5 mg/kg
As: ≤0.1 mg/kg
Cu: ≤5 mg/kg
K: ≤50 mg/kg
Ni: ≤5 mg/kg
Ca: ≤10 mg/kg
Co: ≤5 mg/kg
Cd: ≤5 mg/kg
Pb: ≤5 mg/kg
Bi: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Cr: ≤5 mg/kg
Fe: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Al: ≤5 mg/kg
Impurities
DNases, none detected
insoluble matter, passes filter test
<0.005% formaldehyde (HCHO)
RNases, none detected
≤0.1% biuret
phosphatases, none detected
proteases, none detected
Ignition Residue
≤0.01% (as SO4)
Linear Formula
NH2CONH2
Loss on Drying
≤0.1% loss on drying, 20 °C (HV)
Grade
for molecular biology
Product Line
BioUltra
λ
8 M in H2O
Purity
≥99.5% (T)
Physical Property
pH
8.0-10.0 (20 °C, 8 M in H2O)
Absorption Wavelength
λ: 260 nm Amax: ≤0.03
λ: 280 nm Amax: ≤0.02
Density
1.335 g/mL at 25 °C(lit.)
Melting Point
132-135 °C(lit.)
Solubility
H2O: soluble8 M at 20 °C, clear, colorless
Safety Information
Personal Protective Equipment
Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
German water hazard class
1
MSDS Link
RTECS
YR6250000
Pharmacology Properties
Gene Information
human ... CA1(759), CA2(760)rat ... Ppm1a(24666)
Molecule Details
Application
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.
Other Notes
Denaturing reagent1; Commonly used in conjunction with polyacrylamide gel electrophoresis for the fractionation of single-stranded nucleic acids with chains of up to 200 nucleotides2,3,4
Molecular Spectra
No Data Available
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References
No Data Available
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