Substance
ID:351806
Names and Identifiers
Synonyms
bisBenzimidebisBenzimide H 33342 trihydrochlorideHoechst 333422′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochlorideHOE 33342
IUPAC name
2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1,4-dihydroquinazolin-2-yl]-1,4-dihydroquinazoline trihydrochloride
IUPAC Traditional name
2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1,4-dihydroquinazolin-2-yl]-1,4-dihydroquinazoline trihydrochloride
Registration numbers
CAS Number
MDL Number
Beilstein Number
EC Number
PubChem SID
Properties
Safety Information
European Hazard Symbols
Harmful (Xn)Storage Temperature
2-8°C
GHS Precautionary statements
P261-P280-P305+P351+P338
Risk Statements
22-37/38
MSDS Link
GHS Pictograms
Corrosive to metals, category 1
Skin corrosion, categories 1A,1B,1C
Serious eye damage, category 1
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
Safety Statements
22-36/37
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
GHS Signal Word
Danger
German water hazard class
3
RTECS
DT4200000
GHS Hazard statements
H302-H315-H318-H335
Product Information
Grade
for fluorescence
Empirical Formula (Hill Notation)
C27H28N6O · 3HCl · xH2O
Purity
≥97.0% (HPLC)
Physical Property
Fluorescence
λex 340 nm; λem 510 nm
λex 355 nm; λem 465 nm in TE buffer; DNA
Molecule Details
Frequently Asked Questions
Live Chat and Frequently Asked Questions are available for this Product.
Application
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.Excitation max. = 346 nmEmission max. = 460 nm
bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Other Notes
Suitable for vital DNA staining of a variety of cell types1,2; As a probe of membrane permeability in mammalian cells3
Live Chat and Frequently Asked Questions are available for this Product.
Application
Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.Excitation max. = 346 nmEmission max. = 460 nm
bisBenzimide H 33342 trihydrochloride can be used to study biochemicals and reagents, fluorescent probes, labels, particles and stains, luminescent compounds and detection and wavelength index. bisBenzimide H 33342 trihydrochloride, Hoechst 33342, has been used in a study to investigate the effects of inorganic and organic arsenic compounds on human T-lymphoblastoid leukemia cells. Hoechst 33342 has also been used in a study to examine the effects of 9 calcium antagonists on ABCG2/BCRP-mediated resistance and transport in HeLa and SN-38-resistant HeLa (HeLa/SN100) cells, overexpressing ABCG2/BCRP.
Biochem/physiol Actions
Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.
Other Notes
Suitable for vital DNA staining of a variety of cell types1,2; As a probe of membrane permeability in mammalian cells3
Molecular Spectra
No Data Available
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References
No Data Available
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