Other Notes Aminoglycoside antibiotic. Interaction of antibiotics with functional sites in 16S ribosomal RNA5 Application Spectinomycin is used in antibiotic resistance and sensitivity studies in various species such as Escherichia coli, Borrelia burgdorferi, and Neisseria gonorrhoeae 1,2,3. Biochem/physiol Actions Spectinomycin binds to the 30S subunit of the bacterial ribosome thereby inhibiting protein synthesis 4.