Substance
ID:351239
Names and Identifiers
IUPAC name
(2S,3S)-1,4-disulfanylbutane-2,3-diol
Synonyms
DTT克莱兰氏试剂DL-二硫苏糖醇1,4-二巯基苏糖醇DL-DithiothreitolCleland’s reagentthreo-1,4-Dimercapto-2,3-butanediol
IUPAC Traditional name
dithiothreitol
Registration numbers
MDL Number
Beilstein Number
CAS Number
EC Number
PubChem SID
Properties
Product Information
Cation Traces
Cd: ≤5 mg/kg
Fe: ≤5 mg/kg
Ba: ≤5 mg/kg
Ca: ≤10 mg/kg
Pb: ≤5 mg/kg
Co: ≤5 mg/kg
Zn: ≤5 mg/kg
K: ≤50 mg/kg
Al: ≤5 mg/kg
As: ≤0.5 mg/kg
Bi: ≤5 mg/kg
Mg: ≤5 mg/kg
Li: ≤5 mg/kg
Mn: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Ni: ≤5 mg/kg
Sr: ≤5 mg/kg
Na: ≤100 mg/kg
Mo: ≤5 mg/kg
Impurities
DNases, none detected
phosphatases, none detected
proteases, none detected
≤0.3% 4,5-dihydroxy-1,2-dithiane
insoluble matter, passes filter test
RNases, none detected
Purity
≥99.5% (RT)
Product Line
BioUltra
Grade
for molecular biology
Antion Traces
sulfate (SO42-): ≤50 mg/kg
λ
0.1 M in H2O
Linear Formula
HSCH2CH(OH)CH(OH)CH2SH
Physical Property
pH
4.0-6.5 (25 °C, 0.1 M in H2O)
Flash Point
235.4 °F
113 °C
Solubility
H2O: soluble0.1 M at 20 °C, clear, colorless
Melting Point
41-44 °C(lit.)
Absorption Wavelength
λ: 260 nm Amax: 0.400
λ: 280 nm Amax: 0.100
Safety Information
GHS Signal Word
Warning
Risk Statements
22-36/37/38
European Hazard Symbols
Harmful (Xn)MSDS Link
GHS Hazard statements
H302-H315-H319-H335
Safety Statements
26-36
German water hazard class
3
RTECS
EK1610000
Storage Temperature
2-8°C
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
GHS Precautionary statements
P261-P305+P351+P338
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Molecule Details
Application
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds.1
Molecular Spectra
No Data Available
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References
No Data Available
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