Substance
ID:350153
Names and Identifiers
IUPAC Traditional name
L-methionine sulfoximine
Synonyms
L-S-(3-Amino-3-carboxypropyl)-S-methylsulfoximineL-S-(3-氨基-3-羧丙基)-S-甲基砜亚胺L-蛋氨酸砜亚胺L-Methionine sulfoximine
IUPAC name
(2S)-2-amino-4-[imino(methyl)oxo-λ6-sulfanyl]butanoic acid
Registration numbers
CAS Number
Beilstein Number
MDL Number
PubChem SID
Properties
Safety Information
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
Risk Statements
36/37/38
European Hazard Symbols
Irritant (Xi)GHS Signal Word
Warning
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
German water hazard class
3
GHS Precautionary statements
P261-P305+P351+P338
Storage Temperature
2-8°C
GHS Hazard statements
H315-H319-H335
MSDS Link
Safety Statements
26-36
Physical Property
Melting Point
>210 °C (dec.)(lit.)
Product Information
Quality Level
PREMIUM
Linear Formula
CH3S(O)(=NH)CH2CH2CH(NH2)CO2H
Molecule Details
Frequently Asked Questions
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Application
As a potent inhibitor of glutamine sythetase activity, this reagent has widely been used as a selection agent for plasmid integration in Chinese hamster ovary (CHO) and other mammalian cell lines. The growing demand for high yield cell banks for production of recombinant proteins for therapeutics has resulted in two major systems for selection of stable and active clones, Methotrexate selection of dihydrofolate reductase (DHFR) overexpressing cells and MSX selection of glutamine synthetase overexpressing cells. Cells are grown in the absence of glutamine in the media and inhibition of the endogenous activity of glutamine synthease results in cell death for cells lacking overexpression. The MSX-glutamine synthase selection mechanism provides benefits over that of the Methotrexate-DHFR system in that it typically requires a single amplification step and results in significant reduction of time to produce high stability, highly amplified clones.
Biochem/physiol Actions
Methionine sulfoximine (MSX) increases ornithine decarboxylase activity, decreases the survival rate in a model of transient cerebral ischemia, and inhibits glutamine synthetase activity.
Live Chat and Frequently Asked Questions are available for this Product.
Application
As a potent inhibitor of glutamine sythetase activity, this reagent has widely been used as a selection agent for plasmid integration in Chinese hamster ovary (CHO) and other mammalian cell lines. The growing demand for high yield cell banks for production of recombinant proteins for therapeutics has resulted in two major systems for selection of stable and active clones, Methotrexate selection of dihydrofolate reductase (DHFR) overexpressing cells and MSX selection of glutamine synthetase overexpressing cells. Cells are grown in the absence of glutamine in the media and inhibition of the endogenous activity of glutamine synthease results in cell death for cells lacking overexpression. The MSX-glutamine synthase selection mechanism provides benefits over that of the Methotrexate-DHFR system in that it typically requires a single amplification step and results in significant reduction of time to produce high stability, highly amplified clones.
Biochem/physiol Actions
Methionine sulfoximine (MSX) increases ornithine decarboxylase activity, decreases the survival rate in a model of transient cerebral ischemia, and inhibits glutamine synthetase activity.
Molecular Spectra
No Data Available
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References
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