Substance
ID:349334
Names and Identifiers
IUPAC name
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
IUPAC Traditional name
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
Synonyms
CTBCholera Toxin B subunit
Registration numbers
MDL Number
Properties
Product Information
Quality Level
PREMIUM
Compostion
Protein, ~5% Lowry
Mol. Weight
mol wt ~12 kDa
Impurities
≤0.5% Cholera toxin A subunit (SDS-PAGE)
Purity
≥95% (SDS-PAGE)
Physical Property
Apperance
lyophilized powder
Solubility
H2O: soluble10 mg/mL
Safety Information
MSDS Link
German water hazard class
2
Safety Statements
26-36
Storage Temperature
2-8°C
European Hazard Symbols
Harmful (Xn)Risk Statements
20/21/22-36/37/38-52/53
Molecule Details
Analysis Note
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
Packaging
Package size based on protein content
Reconstitution
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Activity measured by ELISA using ganglioside GM1-coated multiwell plates, rabbit anti-Cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. 50% saturation of binding was achieved with 0.05-1 μg of Cholera toxin B subunit per mL.
Packaging
Package size based on protein content
Reconstitution
When reconstituted with water to a final concentration of 1 mg of CTB per ml, the solution will contain 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3 and 1 mM sodium EDTA.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium azide, and sodium EDTA.
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Molecular Spectra
No Data Available
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References
No Data Available
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