Substance
ID:349333
Names and Identifiers
IUPAC name
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
IUPAC Traditional name
N-[4-chloro-3-(trifluoromethyl)phenyl]-2-ethoxybenzamide
Synonyms
CTBCholera Toxin B subunit
Registration numbers
MDL Number
Properties
Safety Information
Safety Statements
26-36
MSDS Link
German water hazard class
2
European Hazard Symbols
Harmful (Xn)Risk Statements
20/21/22-36/37/38-52/53
Storage Temperature
2-8°C
Product Information
Mol. Weight
mol wt ~12 kDa
Conjugate
FITC conjugate
Extent of Labeling
~1.0 mol FITC per mol protein
Compostion
Protein, ~20% Lowry
Physical Property
Apperance
lyophilized powder
Molecule Details
Analysis Note
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Activity measured by ELISA using ganglioside GM1 coated multiwell plates, rabbit anti-cholera toxin B subunit antibodies, and peroxidase-labeled goat anti-rabbit IgG as the second antibody. 50% saturation of binding was achieved with 0.01-1 μg of the cholera toxin B subunit-FITC conjugate per mL.
Physical form
Lyophilized powder containing Tris buffer salts, sodium chloride, sodium EDTA and sodium azide
Biochem/physiol Actions
The cholera toxin B subunit is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport. Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.
Molecular Spectra
No Data Available
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References
No Data Available
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