Substance
ID:347750
Names and Identifiers
IUPAC Traditional name
β-mercaptoethanol
IUPAC name
2-sulfanylethan-1-ol
Synonyms
2-羟基乙硫醇β-巯基乙醇BME硫代乙二醇2-Mercaptoethanol2-巯基乙醇2-HydroxyethylmercaptanThioethylene glycolβ-Mercaptoethanol
Registration numbers
CAS Number
EC Number
Beilstein Number
MDL Number
PubChem SID
Properties
Product Information
Cation Traces
Bi: ≤0.1 mg/kg
Co: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Cd: ≤0.05 mg/kg
Fe: ≤0.1 mg/kg
Ba: ≤0.1 mg/kg
Cu: ≤0.02 mg/kg
Na: ≤1 mg/kg
Al: ≤0.5 mg/kg
Ca: ≤0.5 mg/kg
Sr: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Ni: ≤0.05 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Pb: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg
Cr: ≤0.02 mg/kg
Concentration
14.3 M (pure liquid)
Impurities
insoluble matter, passes filter test
RNases, none detected
phosphatases, none detected
proteases, none detected
DNases, none detected
λ
0.5 M in H2O
Purity
≥99.0% (GC)
Grade
for molecular biology
Linear Formula
HSCH2CH2OH
Product Line
BioUltra
Safety Information
GHS Pictograms
Hazardous to the aquatic environment
Acute hazard, category1
Chronic hazard, categories 1,2
Corrosive to metals, category 1
Skin corrosion, categories 1A,1B,1C
Serious eye damage, category 1
Respiratory sensitization, category 1
Germ cell mutagenicity, categories 1A,1B,2
Carcinogenicity, categories 1A,1B,2
Reproductive toxicity, categories 1A,1B,2
Specific Target Organ Toxicity – Single exposure, categories 1,2
Specific Target Organ Toxicity – Repeated exposure, categories 1,2
Aspiration Hazard, category 1
Acute toxicity (oral, dermal, inhalation), categories 1,2,3
European Hazard Symbols
Toxic (T)
Nature polluting (N)GHS Hazard statements
H301+H331-H310-H315-H317-H318-H373-H410
Hazard Class
6.1
GHS Signal Word
Danger
RID/ADR
UN 2966 6.1/PG 2
Risk Statements
23/24/25-38-41-43-48/22-50/53
RTECS
KL5600000
Personal Protective Equipment
Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
GHS Precautionary statements
P261-P273-P280-P301+P310-P302+P350-P305+P351+P338
Explode Limits
18 %
Storage Temperature
2-8°C
UN Number
2966
Safety Statements
26-36/37/39-45-60-61
MSDS Link
Packing Group
2
German water hazard class
3
Physical Property
Absorption Wavelength
λ: 260 nm Amax: 1.5
λ: 280 nm Amax: 0.3
Refractive Index
n20/D 1.500(lit.)
n20/D 1.501
Vapor Pressure
1 mmHg ( 20 °C)
Density
1.114 g/mL at 25 °C(lit.)
Vapor Density
2.69 (vs air)
Boiling Point
157 °C(lit.)
Solubility
H2O: soluble0.5 M at 20 °C, clear, colorless
Flash Point
68 °C
154.4 °F
Pharmacology Properties
Target Organ
Heart, Liver
Molecule Details
Application
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds1
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.
Other Notes
Isolation of RNA using guanidinium salts. Inclusion of a reductant enhances denaturation by breaking intramolecular protein disulfide bonds1
Molecular Spectra
No Data Available
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References
No Data Available
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