Substance
ID:181905
Names and Identifiers
IUPAC Traditional name
tetramethylbenzidine
IUPAC name
4-(4-amino-3,5-dimethylphenyl)-2,6-dimethylaniline
Synonyms
TMB Blotting PlusTMB substrateBM blueTMB3,3′,5,5′-四甲基联苯胺3,3′,5,5′-TetramethylbenzidineSure Blue TMB
Registration numbers
CAS Number
MDL Number
Beilstein Number
PubChem SID
Properties
Physical Property
Apperance
tablet
Melting Point
168-171 °C(lit.)
Safety Information
GHS Precautionary statements
P305+P351+P338
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
GHS Signal Word
Warning
GHS Hazard statements
H319
Risk Statements
36
Storage Temperature
2-8°C
German water hazard class
3
European Hazard Symbols
Irritant (Xi)Safety Statements
26
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
Molecule Details
Application
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8
本品为联苯胺1的非致癌性替代品(Ames 试验阴性)，适合作为酶联免疫吸附分析的过氧化物酶底物。底物产生一种可溶性淡蓝色终产物，该产物可通过分光光度法在 370 或 620-650nm 处读取。可用 2M H2SO4 终止 TMB 反应(变成黄色)，并在 450nm 处读取数据。一种灵敏的特异性试剂，用于检测血液、2测定血红蛋白、3,4,5测定过氧化物酶。6,7,8
Molecular Spectra
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References
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