Substance
ID:177670
Names and Identifiers
IUPAC name
(2S,3S)-1,4-disulfanylbutane-2,3-diol
Synonyms
DTT克莱兰氏试剂DL-二硫苏糖醇1,4-二巯基苏糖醇DL-Dithiothreitol
IUPAC Traditional name
dithiothreitol
Registration numbers
MDL Number
Beilstein Number
CAS Number
EC Number
PubChem SID
Properties
Product Information
Antion Traces
sulfate (SO42-): ≤0.005%
Cation Traces
As: ≤0.0001%
Cd: ≤0.0005%
Cr: ≤0.0005%
Mg: ≤0.0005%
Mo: ≤0.0005%
Ba: ≤0.0005%
Co: ≤0.0005%
Li: ≤0.0005%
Pb: ≤0.0005%
Ni: ≤0.0005%
Sr: ≤0.0005%
Al: ≤0.0005%
Bi: ≤0.0005%
K: ≤0.005%
Ca: ≤0.001%
Cu: ≤0.0005%
Fe: ≤0.0005%
Mn: ≤0.0005%
Na: ≤0.1%
Zn: ≤0.0005%
Impurities
≤0.3% 4,5-dihydroxy-1,2-dithiane
Insoluble matter, passes filter test
Purity
≥99.0% (titration)
Quality Level
PREMIUM
Physical Property
Absorption Wavelength
A0.1M/260, H2O ≤0.400
A0.1M/280, H2O ≤0.100
Flash Point
235.4 °F
113 °C
pH
4.0-6.0 (20-25 °C, 0.1 m in H2O)
Solubility
H2O: soluble0.1 M, clear, colorless
Melting Point
41-44 °C(lit.)
Safety Information
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
GHS Precautionary statements
P261-P305+P351+P338
European Hazard Symbols
Harmful (Xn)GHS Hazard statements
H302-H315-H319-H335
Risk Statements
22-36/37/38
RTECS
EK1610000
Storage Temperature
2-8°C
GHS Pictograms
Acute toxicity (oral, dermal, inhalation), category 4
Skin irritation, category 2
Eye irritation, category 2
Skin sensitisation, category 1
Specific Target Organ Toxicity – Single exposure, category 3
German water hazard class
3
GHS Signal Word
Warning
Safety Statements
26-36
Molecule Details
Application
用于将 -SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。
用于将 -SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。
Molecular Spectra
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References
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