A chelating agent (chelating agents) that sequesters a variety of polyvalent cations. It is used in pharmaceutical manufacturing and as a food additive. [PubChem]
Indication
For the reduction of blood levels and depot stores of lead in lead poisoning (acute and chronic) and lead encephalopathy, in both pediatric populations and adults.
Pharmacology
Edetate calcium is a heavy metal chelating agent. The calcium in edetate calcium can be displaced by divalent or trivalent metals to form a stable water soluble complex that can be excreted in the urine. In theory, 1 g of edetate calcium can theoretically bind 620 mg of lead, but in reality only about 5 mg per gram is actually excreted into the urine in lead poisoned patients. In addition to chelating lead, edetate calcium also chelates and eliminates zinc from the body. Edetate calcium also binds cadmium, copper, iron and manganese, but to a much lesser extent than either lead or zinc. Edetate calcium is relatively ineffective for use in treating mercury, gold or arsenic poisoning.
Toxicity
Inadvertent administration of 5 times the recommended dose, infused intravenously over a 24 hour period, to an asymptomatic 16 month old patient with a blood lead content of 56 mcg/dl did not cause any ill effects. Edetate calcium disodium can aggravate the symptoms of severe lead poisoning, therefore, most toxic effects (cerebral edema, renal tubular necrosis) appear to be associated with lead poisoning. Because of cerebral edema, a therapeutic dose may be lethal to an adult or a pediatric patient with lead encephalopathy. Higher dosage of edetate calcium disodium may produce a more severe zinc deficiency.
Affected Organisms
Humans and other mammals
Biotransformation
Almost none of the compound is metabolized.
Absorption
Poorly absorbed from the gastrointestinal tract. Well absorbed following intramuscular injection.
Half Life
The half life of edetate calcium disodium is 20 to 60 minutes.
Elimination
It is excreted primarily by the kidney, with about 50% excreted in one hour and over 95% within 24 hours.2 Almost none of the compound is metabolized.
Application A chelator used to remove calcium from cell washing and suspension media. Reduces cell clumping. Physical form Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium
Application Calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. Packaging 25, 100 g in poly bottle
Application Calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. General description Visit our Titration Center to learn more.
Application Calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. Legal Information TraceSELECT is a registered trademark of Sigma-Aldrich Co. LLC
General description EDTA has been titrated to pH 8.0 with NaOH Application Ethylenediaminetetraacetic acid solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, chelators and ethylenediaminetetraacetic acid (EDTA) and EDTA solutions. Ethylenediaminetetraacetic acid solution has been used in a study to help elucidate the role of antigen retrieval in immunostaining of ethanol-fixed smears. Ethylenediaminetetraacetic acid solution has also been used in a study to assess the uptake and release of biotin-labeled fluorescein (b-FITC) as well as immobilization of biotin-labeled glucose oxidase (b-GOx) to the polyelectrolyte microcapsules with an avidin-poly(styrene sulfonate) (PSS) membrane.
Application Calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. Other Notes Determination of anions and metal cations by single-column chromatography with EDTA as eluent using conductivity and UV detection 1; Buffer component in DNA digest analysis with capillary electrophoresis 2
Application Calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. General description Visit our Titration Center to learn more.
Ethylenediamine-N,N,N’,N’-tetraacetic Acid (EDTA) is a powerful chelating agent; EDTA forms stable complexes with most metal ions. EDTA is used in treatment of lead and heavy metal poisoning of farm animals.
References
PubChem Literature
From Data Sources
• Grier, M.T., et al.: Ann. Pharmacother., 27, 1504 (1983)
Nature polluting (N)
Irritant (Xi)