Molecule

02150629 

Purity: 97%
Off-white to tan crystals.

02152347 

Stabilized Chromogen Chromogen solution for immunohistology procedures and peroxidase and pseudo-peroxidase reactions. Ready-to-use directly as the final step in developing the color indicator in the enzyme reaction. Precipitates as a readily detected marker for immunohistopathological staining. Stable up to 15 months.

05215236 

MP Biomedicals Rare Chemical collection

C8302 

Other Notes
0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.
Substrates
Substrate for visualizing peroxidase conjugates in Western blotting.

317403 

Application
Peroxidase stain.1

C57804 

Packaging
25 g in glass bottle

C8890 

Packaging
2.5, 5, 25, 100 g in glass bottle
Substrates
Chromogenic peroxidase substrate useful in enzyme-linked detection procedures.

C6788 

Quantity
Contains 30 mg substrate per tablet.
Reconstitution
Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.
Specificity
4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

25328 

Application
electrophoresis test: suitable for peroxidase detection on blotting membranes
Other Notes
Peroxidase stain. In the detection of specific antigens after isoelectric focusing and immunoblotting into nitrocellulose membranes1; In a dot-immunobinding assay for the detection and quantitation of human IgE2; A rapid multicolor Western blot3