Molecule

S1677 

Research Area: Infection
Biological Activity:
Chloramphenicol(Chloromycetin) is a bacteriostatic antimicrobial. It is considered as a prototypical broad-spectrum antibiotic. It is effective against a wide variety of Gram-positive and Gram-negative bacteria, including most of anaerobic organisms. It is bacteriostatic (that is, it stops bacterial growth). It is a protein synthesis inhibitor, inhibiting peptidyl transferase activity of the bacterial ribosome, binding to A2451 and A2452 residues in the 23S rRNA of the 50S ribosomal subunit, preventing peptide bond formation. [1]

05204959 

MP Biomedicals Rare Chemical collection

Chloramphenicol 

C1919 

Application
Used as a seletion agent for transformed cells containing chloramphenicol resistance genes.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

C1863 

Application
Used as a seletion agent for transformed cells containing chloramphenicol resistance genes.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

C0857 

Application
Used as a seletion agent for transformed cells containing chloramphenicol resistance genes. Recommended for use in cell culture applications at 10 ml/L.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).

C0378 

Application
Used as a seletion agent for transformed cells containing chloramphenicol resistance genes.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Packaging
1 kg in poly bottle
5, 25, 100, 500 g in poly bottle
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

C7795 

Application
Use as a seletion agent for transformed cells containing chloramphenicol resistance genes. Recommended for use in molecular biology applications at 10-20 μg/ml.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Reconstitution
Prepare stock solutions directly in the vial at any concentration in the recommended range. Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 5 days.
Protocols & Applications
Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information

23275 

Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
Other Notes
Inhibits protein biosynthesis by binding to the large subunit of the bacterial ribosome and by blocking the peptidyl transfer; chloramphenicol-resistance is a useful genetic marker for the selection of eukaryotic cells;1 Resistance is usually mediated by a chloramphenicol-acetyl-transferase, but there are exceptions;2 Review.3

31667 

Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
General description
Standard for Supelco MIP SPE cartridges. For more information request Supelco Literature T407075, T706024
Legal Information
VETRANAL is a trademark of Sigma-Aldrich Co. LLC

23276 

Application
Used as a seletion agent for transformed cells containing chloramphenicol resistance genes.
Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).

442513 

Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).

46110 

Biochem/physiol Actions
Mode of Action: Inhibits translation on the 50S ribosomal subunit at the peptidyltransferase step (elongation inhibition). Bacteriostatic.Mode of Resistance: Acetylation by chloramphenicol acetyltransferase (cat gene).
General description
Standard for Supelco MIP SPE cartridges. For more information request Supelco Literature T407075, T706024
Legal Information
VETRANAL is a trademark of Sigma-Aldrich Co. LLC

C325030 

Broad spectrum antibiotic obtained from cultures of the soil bacterium Streptomyces venezuelae. It has a broad spectrum of activity against Gram-positive and gram-negative bacteria. Antibacterial; antirickettsial.