Molecule

02194956 

Hydrochloride
Purity: >75%
A stable, non-toxic alternative to DFP or PMSF. Specifically and irreversibly inhibits trypsin-like serine proteases, trypsin, and thrombin. Demonstrates activity on factor Xa, plasmin, C1r and C1s. Activity is 1000 fold greater than PMSF.

06566 

Other Notes
An irreversible inhibitor of serine proteases4
Application
APMSF was used to study the glucagon-receptor interaction; inhibition of cAMP formation led to the conclusion that there was a previously unexpected serine residue in the active site which may operate on the receptor by a mechanism similar to serine proteases.1
Biochem/physiol Actions
Irreversible inhibitor of serine proteases with lysine or arginine substrate specificities. Effective concentration 10-100 μM. Frequently used to characterize newly discovered proteases.2,3