CAS 102185-33-1|5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALT|disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate|dipotassium 5-bromo-4-chloro-1H-indol-3-yl phosphate|BCIP|X-phosphate|5-Bromo-4-c...

General Information

Structure

Molecular Formula

C₈H₄BrClNNa₂O₄P

Molecular Mass

370.431961

Exact Mass

368.85452249

Charge

InChI

InChI=1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2

InChIKey

OAZUOCJOEUNDEK-UHFFFAOYSA-L

Canonic Smiles

Clc1c(Br)ccc2c1c(c[nH]2)OP(=O)([O-])[O-].[Na+].[Na+]

Isomeric Smiles

[Na+].[Na+].[O-]P(=O)([O-])Oc1c[nH]c2c1c(Cl)c(Br)cc2

Calculated Properties

JChem

Acid pKa

1.726015

H Acceptors

H Donor

LogD (pH = 5.5)

0.10124553

LogD (pH = 7.4)

-0.6621499

Log P

2.4873745

Molar Refractivity

60.1825

Polarizability

25.167912

Polar Surface Area

88.21

Rotatable Bonds

Lipinski's Rule of Five

true

Data Source

Commercial Catalog

Academic Data

Data Source

Names and Identifiers

Synonyms

5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALTX-phosphateBCIP5-Bromo-4-chloro-3-indolyl phosphate disodium saltBCIP®X-phosphate disodium salt5-溴-4-氯-3-吲哚磷酸二钠盐

IUPAC name

disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate

IUPAC Traditional name

dipotassium 5-bromo-4-chloro-1H-indol-3-yl phosphate disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate

Names and Identifiers

Registration numbers

CAS Number

MDL Number

PubChem SID

PubChem CID

Registration numbers

Properties

Safety Information

Storage Condition

-20°C, Desiccate, Protect from light

0°C, Desiccate, Protect from light

MSDS Link

Download link

Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter

-20°C

Physical Property

Melting Point

> 300°C

Solubility

H2O: soluble20 mg/mL

DMF: insoluble

Apperance

powder

Properties

Related Proteins

No Data Available

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Molecular Spectra

No Data Available

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Molecule Details

MP Biomedicals

02150042

Substrate for alkaline phosphatase
Purity: 99%

02193989

Molecular Biology Reagent
Disodium Salt
Purity: >98%
Chromogenic substrate for alkaline phosphatase in ELISA.

Sigma Aldrich

B1026

Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
法律信息
BCIP 注册商标 Sigma-Aldrich Co. LLC

16668

Other Notes
For visualizing antigenic proteins immobilized on nitrocellulose paper1,2
Substrates
Histochemical substrate for alkaline phosphatase.
Application
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
Legal Information
BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Molecule Details

References

PubChem Literature

No Data Available

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References

Bioactivity

PubChem BioAssay

Bioactivity

Data Source

Commercial Catalog

Academic Data

Names and Identifiers

Synonyms

5-BROMO-4-CHLORO-3-INDOLYL PHOSPHATE DISODIUM SALTX-phosphateBCIP5-Bromo-4-chloro-3-indolyl phosphate disodium saltBCIP®X-phosphate disodium salt5-溴-4-氯-3-吲哚磷酸二钠盐

IUPAC name

disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate

IUPAC Traditional name

dipotassium 5-bromo-4-chloro-1H-indol-3-yl phosphate disodium 5-bromo-4-chloro-1H-indol-3-yl phosphate

Registration numbers

CAS Number

102185-33-1

MDL Number

MFCD00036757

PubChem SID

162089603

PubChem CID