Application The two isomers are produced by the usual synthesis. Most publications imply they are interchangeable when used for the preparation of the isothiocyanate for fluorescent antibody work. In fact, most commercial preparations are not even labeled as to whether or not they are mixtures. Other Notes All known glycosaminoglycuronans react with this fluorescein derivative to yield fluorescent derivatives1 Note: Do not confuse with fluorescamine.
A primary amino compound that is fluorescein carrying an amino substituent at C-5. Building block/intermediate for the synthesis of the fluorescent dye flourescein; also used to produce N-(fluorescein-5-yl)maleamic acid.
References
PubChem Literature
From Data Sources
• Mann, K.G. and Fish, W.W., Methods in Enz., 26, 28 (1958)
• Riggs, H.J.L., et al.: Amer. J. Pathol., 34, 1081 (1958)